The Agazie lab focuses on the role of the Src homology phosphotyrosyl phosphatase 2 (SHP2) in receptor tyrosine kinase and the Wnt/ ß- catenin signaling pathways and its role in cancer.
Dr. Bobko is interested in developing of new probes and approaches for in vivo multifunctional spectroscopy and imaging using electron and nuclear magnetic resonance techniques. We focus on synthesis of paramagnetic probes for measurement of tissue microenvironment parameters (e.g. acidity (pH), redox, glutathione (GSH), inorganic phosphate (Pi), glucose, oxygenation (pO2)) using Electron Paramagnetic Resonance (EPR) or/and Nuclear Magnetic Resonance (NMR) spectroscopy and imaging and their combinations.
Our lab integrates multidisciplinary approaches including mass spectrometry, stable isotope tracers, gene editing, animal models and stem cell technology to study the roles of metabolic regulation and dys-regulation in the heathy and diseased retinas.
Most of my research is devoted to the development and application of new magnetic resonance approaches to biomedicine, including electron paramagnetic resonance (EPR) spectroscopy and imaging and Overhauser-enhanced magnetic resonance imaging (OMRI or proton-electron double-resonance imaging, PEDRI). Our current projects develop the unique paramagnetic probes allowing noninvasive simultaneous detection of tissue oxygenation, acidity (pH), redox status, intracellular glutathione (GSH) and interstitial inorganic phosphate in living subjects.
Our lab is interested in the development of new strategies to manipulate the metabolic network for the treatment or prevention of metabolic disorders like type 2 diabetes. In particular, the lab focuses on coenzyme A (CoA)-an essential cofactor that acts as a global regulator of cellular metabolism- and on the role of CoA-degrading enzymes in the regulation of CoA levels and energy metabolism. We are also interested in understanding, at a mechanistic level, the connection between neuronal CoA levels, neurodegeneration and brain iron accumulation in PKAN disease to identify new therapeutic targets for this neurological disorder.
The fundamental question we are interested in is how cell adhesion and cell mitotic machineries communicate with each other. It is the matter of life for a multi-cellular organism, where specific and oriented adhesions were evolutionary necessary to develop. The focus of the Pugacheva Lab is the focal adhesion scaffolding proteins of the Cas family and their role in proliferation and invasion. Our current efforts are dedicated to outlining the molecular mechanisms governing Cas dependent activation of oncogenic kinase AurA and finding AurA substrates responsible for tumor progression.
Dr. Rajendran is interested in investigating the electrolyte transport processes that regulate colonic fluid movement during physiological and pathophysiological (diarrhea and ulcerative colitis) conditions. We focus to identify the Ca2+-activated intermediate conductance (also known as KCNN4) K+ channel isoform that provides the driving force for Cl- secretion in several fluid secreting epithelial cells. To achieve this goal, we employ electrophysiology, biochemical, molecular and biophysical techniques.
Biochemical mechanisms behind gene mutations that result in photoreceptor cell death; Protein methylation in neurons; Gene therapy for blinding diseases.
Non-coding RNAs play diverse cellular roles acting as messengers, regulators, structural scaffolds, and catalytic ribozymes. We use a combination of biochemistry and structural biology to understand the architecture and catalytic mechanisms of RNA molecular machines, exploring the complexity of RNA structure and the proteins that coordinate to them. The lab is currently focused on two RNA machines: the RNA splicing apparatus and the telomerase ribonucleoprotein (RNP) complex.
My lab's passion is to understand how the proteasome functions at a molecular level. The proteasome is a molecular machine that finds proteins, unfolds them, and injects them into an internal chamber were they are destroyed. This protein degradation process plays important roles in the development of neurodegenerative diseases (e.g. Alzheimer’s) and cancer. Understanding these disease and molecular mechanisms is also allowing us to develop new proteasome modulating compounds that could be useful to treat these diseases.
Regulation of alternative pre-mRNA splicing; Alternative splicing in cancer progression; Drugs targeting alternative splicing as cancer therapeutics and research tools; High-throughput research methods.
My collaborators and I are developing new spectroscopic and imaging methods for in vivo Electron Paramagnetic Resonance (EPR). EPR is in many ways analogous to better known Nuclear Magnetic Resonance (NMR) and its imaging modality MRI. While NMR and MRI methods detect signals produced by nuclear spins in protons and other chemical elements, EPR measures electron spins in all kinds of free radicals.